detail view molecular characterisation GMO Detection Methods

  • GMO:

    5715
  • Alias:

    Cibus Canola Event 5715, GE canola, GE rape, gene editing, Herbicide tolerant canola
  • UID:

  • Tradename:

    SU Canola
  • Company:

    Cibus US LLC
  • Developer:

    Cibus US LLC
  • Species:

    Brassica napus
    (canola, oilseed rape, rapeseed)
  • Traits:

      Herbicide tolerance > ALS/AHAS inhibitor tolerance > Imidazolinone tolerance

      Herbicide tolerance > ALS/AHAS inhibitor tolerance > Sulfonylurea tolerance

  • EU authorisation:

    not approvednot approvednot approvednot approved
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NameSort Icon TypeSort Icon Target DescriptionSort Icon ValidationSort Icon StandardisationSort Icon Verification
QT-TAX-BN-001 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method 1
QT-TAX-BN-012 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method 1
QT-TAX-BN-003 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) ring trial validation EU reference method 1
QT-TAX-BN-002 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4

ring trial validation EU reference method 1
QT-TAX-FatA primer1/FatA primer2 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

in-house validation none 1
QL-TAX-BN-004 taxon-specific
Brassica napus
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). ring trial validation unknown 1
QL-TAX-BN-003 taxon-specific
Brassica napus
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) ring trial validation unknown 1
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