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Detection Image Detection method details

Name:

 QT-TAX-ST-010

Description:

 Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)

Comment:

 Target is the endogenous UDP-glucose pyrophosphorylase (UGPase) gene of Solanum tuberosum

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Solanum tuberosum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    UGP-af7

  • Sequence:

    GGACATGTGAAGAGACGGAGC

  • Size:

    21

  • Reverse Primer

  • Name:

    UGP-ar8

  • Sequence:

    CCTACCTCTACCCCTCCGC

  • Size:

    19

  • Probe

  • Name:

    UGP-sf1

  • Sequence:

    CTACCACCATTACCTCGCACCTCCTCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    ggacatgtgaagagacggagcnnnnnnnnnnnnnntgaggaggtgcgaggtaatggtggtagnnnnnnngcnggaggggtagaggtagg

  • Size:

    89

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-ST-010Link to the document url. EU reference method protocol