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Detection Image Detection method details

Name:

QT-TAX-GH-015

Description:

Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

Comment:

Target is the fiber-specific acyl carrier protein (ACP1) gene from Gossypium hirsutum.

This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region.

Validation:

ring trial validation

Standardisation:

EU reference method

Related Methods:

Type:

taxon-specific

Target Species name:

Gossypium hirsutum
  • Oligonucleotides:

  • Forward Primer

  • Name:

    primer forward
  • Sequence:

    ATTGTGATGGGACTTGAGGAAGA
  • Size:

    23
  • Reverse Primer

  • Name:

    primer reverse
  • Sequence:

    CTTGAACAGTTGTGATGGATTGTG
  • Size:

    24
  • Probe

  • Name:

    probe
  • Sequence:

    ATTGTCCTCTTCCACCGTGATTCCGAA
  • Size:

    27
  • Amplicon:

  • Sequence:

    attgtgttgggacttgaggaaganttcggaatcacggtggaagaggacaacncacaatccatcacaactgttcaag
  • Size:

    76

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article 31-10-2018
EU-RL GMFF GMOMETHODS database; QT-TAX-GH-015Link to the document url. EU reference method protocol