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Detection Image Detection method details

Name:

 QT-EVE-ZM-020

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event MON 810 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON810

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Mail-F1

  • Sequence:

    TCGAAGGACGAAGGACTCTAACGT

  • Size:

    24

  • Reverse Primer

  • Name:

    Mail-R1

  • Sequence:

    GCCACCTTCCTTTTCCACTATCTT

  • Size:

    24

  • Probe

  • Name:

    mail-S2

  • Sequence:

    AACATCCTTTGCCATTGCCCAGC

  • Size:

    23

  • Amplicon:

  • Sequence:

    tcgaaggacgaaggactctaacgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaagatagtggaaaaggaaggtggc

  • Size:

    92

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-020Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document