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Detection Image Detection method details

Name:

 QT-EVE-ZM-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event VCO-01981-5 (EURL GMFF GMOMETHODS database as of July 14, 2016)

Comment:

 Target is 5' integration border region (IBR) between the insert of maize event VCO-01981-5 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-004

Type:

 event-specific

Target GMO name:

 VCO1981

  • Oligonucleotides:

  • Forward Primer

  • Name:

    VCO-01981-5 primer F

  • Sequence:

    CCACTgAACgTCACCAAgAAgA

  • Size:

    22

  • Reverse Primer

  • Name:

    VCO-01981-5 primer R

  • Sequence:

    gCCgCTACTCgAgggATTTA

  • Size:

    20

  • Probe

  • Name:

    VCO-01981-5 probe

  • Sequence:

    CAgTACTCAAACACTgATAg

  • Size:

    20

  • Amplicon:

  • Sequence:

    ccactgaacgtcaccaagaagancagtactcaaacactgatagnnnnnnnnnnnnnnnnnnnnnntaaatccctcgagtagcggc

  • Size:

    85

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