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Detection Image Detection method details

Name:

QT-EVE-GM-020

Description:

Quantitative real-time PCR (TaqMan) method for detection of soybean event MON94313 (EU-RL GMFF GMOMETHODS database as of November 29, 2024)

Comment:

Target is the 5' integration border region (IBR) between the insert of soybean event MON94313 and the soybean host genome.

Validation:

ring trial validation

Standardisation:

EU reference method

Related Methods:

QL-TAX-GM-002

Type:

event-specific

Target GMO name:

MON94313
  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON 94313 primer 1
  • Sequence:

    GCTTTAATCCATCGTCCCTTAGTC
  • Size:

    24
  • Reverse Primer

  • Name:

    MON 94313 primer 2
  • Sequence:

    GGCCTAACTTTTGGTGTGATGAT
  • Size:

    23
  • Probe

  • Name:

    MON 94313 probe
  • Sequence:

    CTGACTGGCTACAAGAC
  • Size:

    17
  • Amplicon:

  • Sequence:

    GCTTTAATCCATCGTCCCTTAGTCNNNNNGTCTTGTAGCCAGTCAGNATCATCACACCAAAAGTTAGGCC
  • Size:

    70

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-020Link to the document url. EU reference method protocol