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Detection Image Detection method details

Name:

 QT-EVE-GH-005

Description:

 Quantitative real-time PCR (TaqMan) method for the detection of cotton event MON15985 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of cotton event MON15985 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-015

Type:

 event-specific

Target GMO name:

 MON15985

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON15985 primer 1

  • Sequence:

    GTTACTAGATCGGGGATATCC

  • Size:

    21

  • Reverse Primer

  • Name:

    MON15985 primer 2

  • Sequence:

    AAGGTTGCTAAATGGATGGGA

  • Size:

    21

  • Probe

  • Name:

    MON15985 Probe

  • Sequence:

    CCGCTCTAGAACTAGTGGATCTGCACTGAA

  • Size:

    30

  • Amplicon:

  • Sequence:

    gttactagatcggggatatccnnnnnnnnnccgctctagaactagtggatctgcactgaantcccatccatttagcaacctt

  • Size:

    82

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-005Link to the document url. EU reference method protocol