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Detection Image Detection method details

Name:

 QT-EVE-BV-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of sugar beet event KWS20-1 (EU-RL GMFF GMOMETHODS database as of November 29, 2024).

Comment:

 Target is the 3' integration border region (IBR) between the insert of sugar beet event KWS20-1 and the sugar beet host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BV-001

Type:

 event-specific

Target GMO name:

 KWS20-1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    2109_fwd1

  • Sequence:

    TGTCGTTTCCCGCCTTC

  • Size:

    17

  • Reverse Primer

  • Name:

    2109_rev1

  • Sequence:

    TCCTACCAATTCTTGAACTTCGTG

  • Size:

    24

  • Probe

  • Name:

    2109_Probe 1

  • Sequence:

    ACTATCAGTGTTTCAT

  • Size:

    16

  • Amplicon:

  • Sequence:

    TGTCGTTTCCCGCCTTCNNNNNNNACTATCAGTGTTTCATNNNNNNNNNNNNNCACGAAGTTCAAGAATTGGTAGGA

  • Size:

    77

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BV-002Link to the document url. EU reference method protocol