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Detection Image Detection method details

Name:

 QT-EVE-BN-010

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of oilseed rape event MON88302 and the oilseed rape host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-002

Type:

 event-specific

Target GMO name:

 MON88302

  • Oligonucleotides:

  • Forward Primer

  • Name:

    88302QF

  • Sequence:

    TCCTTGAACCTTATTTTATAGTGCACA

  • Size:

    27

  • Reverse Primer

  • Name:

    88302QR

  • Sequence:

    TCAGATTGTCGTTTCCCGCCTTCA

  • Size:

    24

  • Probe

  • Name:

    88302QP

  • Sequence:

    TAGTCATCATGTTGTACCACTTCAAACACT

  • Size:

    30

  • Amplicon:

  • Sequence:

    tccttgaaccttattttatagtgcacannnnnnnntagtcatcatgttgtaccacttcaaacactnnnnnnnnnnnntgaaggcgggaaacgacaatctga

  • Size:

    101

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-010Link to the document url. EU reference method protocol