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Detection Image Detection method details

Name:

 QT-ELE-P35S 1-5'/P35S 1-3'

Description:

 Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)

Comment:

 target is the 35S promoter (P-35S) sequence from the Cauliflower mosaic virus (CaMV)

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 element-specific

Target DNA element:
P-Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    P35S 1-5'

  • Sequence:

    ATTGATGTGATATCTCCACTGACGT

  • Size:

    25

  • Reverse Primer

  • Name:

    P35S 1-3'

  • Sequence:

    CCTCTCCAAATGAAATGAACTTCCT

  • Size:

    25

  • Probe

  • Name:

    P35S-Taq

  • Sequence:

    CCCACTATCCTTCGCAAGACCCTTCCT

  • Size:

    27

  • Amplicon:

  • Sequence:

    CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAT

  • Size:

    100

Documents

Citation Type Local copy
Kuribara, H., Shindo, Y., Matsuoka, T., Takubo, K., Futo, S., Aoki, N., et al. (2002). Novel reference molecules for quantitation of genetically modified maize and soybean. Journal of the AOAC International, 85, 1077–1089.Link to the document url. peer-reviewed article