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Detection Image Detection method details

Name:

 QT-ELE-NOS ter 2-5' /NOS ter 2-3'

Description:

 Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)

Comment:

 target is the nopaline synthase terminator (T-nos) from bacterium Agrobacterium tumefaciens. Amplicon was generated from cotton LLcotton25 (GenBank HQ233646.1)

Validation:

 ring trial validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    NOS ter 2-5'

  • Sequence:

    GTCTTGCGATGATTATCATATAATTTCTG

  • Size:

    29

  • Reverse Primer

  • Name:

    NOS ter 2-3'

  • Sequence:

    CGCTATATTTTGTTTTCTATCGCGT

  • Size:

    25

  • Probe

  • Name:

    NOS-Taq

  • Sequence:

    AGATGGGTTTTTATGATTAGAGTCCCGCAA

  • Size:

    30

  • Amplicon:

  • Sequence:

    GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCG

  • Size:

    151

Documents

Citation Type Local copy
Kuribara, H., Shindo, Y., Matsuoka, T., Takubo, K., Futo, S., Aoki, N., et al. (2002). Novel reference molecules for quantitation of genetically modified maize and soybean. Journal of the AOAC International, 85, 1077–1089.Link to the document url. peer-reviewed article