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Detection Image Detection method details

Name:

 QT-CON-00-007

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002)

Comment:

 Target is the junction region between a synthetic cryIA(b) gene and the phospho-enol-pyruvate carboxylase (PEPC) intron N.9

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QT-TAX-ZM-006

Type:

 construct-specific

Target GMO names:
B827, Bt176

  • Oligonucleotides:

  • Forward Primer

  • Name:

    E176 2-5'

  • Sequence:

    TGTTCACCAGCAGCAACCAG

  • Size:

    20

  • Reverse Primer

  • Name:

    E176 2-3'

  • Sequence:

    ACTCCACTTTGTGCAGAACAGATCT

  • Size:

    25

  • Probe

  • Name:

    E176-Taq

  • Sequence:

    CCGACGTGACCGACTACCACATCGA

  • Size:

    25

  • Amplicon:

  • Sequence:

    tgttcaccagcagcaaccagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagatctgttctgcacaaagtggagt

  • Size:

    100

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-CON-00-007Link to the document url. EU reference method protocol
Shindo, Y., Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J., Akiyama, H., Goda, Y., Toyoda, M., Hino, A. (2002). Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. Journal of the AOAC International, 85, 1119-1126Link to the document url. peer-reviewed article
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document