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Detection Image Detection method details

Name:

 QT-CON-00-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO 21570)

Comment:

 Target is the junction region between the chloroplast transit peptide (CTP) sequence from Petunia hybrida epsps gene and the CP4 epsps gene from Agrobacterium tumefaciens

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QT-TAX-GM-007

Type:

 construct-specific

Target GMO names:
GTS 40-3-2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RRS 01-5'

  • Sequence:

    CCTTTAGGATTTCAGCATCAGTGG

  • Size:

    24

  • Reverse Primer

  • Name:

    RRS 01-3'

  • Sequence:

    GACTTGTCGCCGGGAATG

  • Size:

    18

  • Probe

  • Name:

    RRS-Taq

  • Sequence:

    CGCAACCGCCCGCAAATCC

  • Size:

    19

  • Amplicon:

  • Sequence:

    cctttaggatttcagcatcagtggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncattcccggcgacaagtc

  • Size:

    121

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-CON-00-001Link to the document url. EU reference method protocol
Shindo, Y., Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J., Akiyama, H., Goda, Y., Toyoda, M., Hino, A. (2002). Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. Journal of the AOAC International, 85, 1119-1126Link to the document url. peer-reviewed article
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document