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Detection Image Detection method details

Name:

 QL-EVE-MS-J101-F/J101-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J101 (Guertler et al. 2018)

Comment:

 Target is the 5' integration border region (IBR) between the insert of alfalfa event J101 and the alfalfa host genome

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QL-TAX-Acc1-F/Acc1-R

Type:

 event-specific

Target GMO name:

 J101

  • Oligonucleotides:

  • Forward Primer

  • Name:

    J101-F

  • Sequence:

    gTCATgTgTTTTgTACTgATCTTgTg

  • Size:

    26

  • Reverse Primer

  • Name:

    J101-R

  • Sequence:

    gACCTgCAgAAgCTTgATgg

  • Size:

    20

  • Probe

  • Name:

    J101-P

  • Sequence:

    ACTgAAggCgggAAACgACAATCTgATCC

  • Size:

    29

  • Amplicon:

  • Sequence:

    GTCATGTGTTTTGTACTGATCTTGTGTCATAGTTTCAAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCCCCATCAAGCTTCTGCAGGTC

  • Size:

    102

Documents

Citation Type Local copy
Guertler et al., Development of event-specific qPCR detection methods for genetically modified alfalfa events J101, J163 and KK179. Biomolecular Detection and Quantification 2019, 17, 100076.Link to the document url. peer-reviewed article