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Detection Image Detection method details

Name:

 QL-EVE-KP-001

Description:

 Qualitative real-time PCR (TaqMan) method for detection of Komagataella phaffii event MxY0541 (EU-RL GMFF GMOMETHODS database as of December 18, 2024).

Comment:

 Target is the 3' integration border region (IBR) between the insert of Komagataella phaffii event MxY0541 and the Komagataella phaffii host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 MXY0541 Pichia pastoris

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MxY0541-F10

  • Sequence:

    CTGCATCTCTCAGGCAAATGGC

  • Size:

    22

  • Reverse Primer

  • Name:

    MxY0541-R10

  • Sequence:

    AACTCACTTCCGTACGCAACCA

  • Size:

    22

  • Probe

  • Name:

    MxY0541-P10

  • Sequence:

    TCCTCTTGAGCTAGCTACCGT

  • Size:

    21

  • Amplicon:

  • Sequence:

    AACTCACTTCCGTACGCAACCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGTAGCTAGCTCAAGAGGANNNNNNNNNGCCATTTGCCTGAGAGATGCAG

  • Size:

    130

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-EVE-KP-001Link to the document url. EU reference method protocol