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Detection Image Detection method details

Name:

 QL-ELE-Vip3A-F/Vip3A-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2014).

Comment:

 Target is the vip3A gene from bacterium Bacillus thuringiensis strain AB88.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-vip3Aa20-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Vip3A-F

  • Sequence:

    TCACCAAGAAGATGAAGAC

  • Size:

    19

  • Reverse Primer

  • Name:

    Vip3A-R

  • Sequence:

    CTCTCCACCTTCTTCTTG

  • Size:

    18

  • Probe

  • Name:

    Vip3A-P

  • Sequence:

    TGACCGCCAACTTCTACGACA

  • Size:

    20

  • Amplicon:

  • Sequence:

    -

  • Size:

    97

Documents

Citation Type Local copy
Liang, C., van Dijk, J.P., Scholtens, I.M.J., Staats, M., Prins, T.W., Voorhuijzen, M.M., da Silva, A.M., Maisonnave Arisi, A.C., den Dunnen, J., Kok, E.J. (2014) Detecting authorized and unauthorized genetically modified organisms containing vip3A by real-time PCR and next-generation sequencing. Anal Bioanal Chem 406, 2603-261Link to the document url. peer-reviewed article