Detection method details
Name: |
QL-ELE-T-g7-F/T-g7-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013; Official Collection of Methods according to § 28b GenTG, G 30.40-22) |
Comment: |
Target is the Agrobacterium tumefaciens g7 terminator (T-g7)
T-g7 (aka T-transcript7 or T-tr7) also recognises ORF23 of Agrobacterium tumefaciens since this is encoded by the reverse strand of T-g7. As described in G 30.40-22, the PCR was optimised by modifying the chemistry (in particular, by introducing the use of an internal quencher in the probe). |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-transcript 7, T-orf23-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
T-g7-FSequence:
ATGCAAGTTTAAATTCAGAAATATTTCAASize:
29
Reverse Primer
Name:
T-g7-RSequence:
ATGTATTACACATAATATCGCACTCAGTCTSize:
30
Probe
Name:
T-g7-PSequence:
ACTGATTATATCAGCTGGTACATTGCCGTAGATGASize:
35
-
Amplicon:
Sequence:
-Size:
97
Documents
| Citation | Type | Local copy |
|---|---|---|
Detection of T-E9 and T-g7 terminator sequences for screening genetically modified plants using real-time PCR (singleplex) |
other document |