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Detection Image Detection method details

Name:

 QL-ELE-T-OCS-F/T-OCS-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013)

Comment:

 Target is the octopine synthase terminator (T-OCS) from Agrobacterium tumefaciens.

There are two nucleotides missing in the reverse primer. The amplicon given is the actual sequence generated from Agrobacterium tumefaciens (GenBank: CP011249.1) T-OCS-R: CGCTCGGTGTCGTAGATACT (Debode et al., 2013) T-OCS-R: CGCTCGGTGTGTCGTAGATACT (corrected)

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
T-ocs-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    T-OCS-F

  • Sequence:

    CGGTCAAACCTAAAAGACTGATTACA

  • Size:

    26

  • Reverse Primer

  • Name:

    T-OCS-R

  • Sequence:

    CGCTCGGTGTCGTAGATACT

  • Size:

    20

  • Probe

  • Name:

    T-OCS-P

  • Sequence:

    TCTTATTCAAATTTCAAAAGTGCCCCAGGG

  • Size:

    30

  • Amplicon:

  • Sequence:

    CGGTCAAACCTAAAAGACTGATTACATAAATCTTATTCAAATTTCAAAAGTGCCCCAGGGGCTAGTATCTACGACACACCGAGCG

  • Size:

    85

Documents

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