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Detection Image Detection method details

Name:

 QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD).

Comment:

 Target is the cry3Bb gene from bacterium Bacillus thuringiensis subsp. kumamotoensis.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry3Bb1-BACUK

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Cry3Bbf-n2

  • Sequence:

    CCGCCCAGGACTCCATCG

  • Size:

    18

  • Reverse Primer

  • Name:

    Cry3Bbr-n2

  • Sequence:

    GAGGCACCCGAGGACAGG

  • Size:

    18

  • Probe

  • Name:

    Cry3BbP-n3

  • Sequence:

    CTGCCGCCTGAGACCACTGACGAGC

  • Size:

    25

  • Amplicon:

  • Sequence:

    ccgcccaggactccatcgaccagctgccgcctgagaccactgacgagcccctggagaaggcctactcccaccagctgaactacgcggagtgcttcctgatgcaagaccgcaggggcaccatccccttcttcacctggacccaccgctccgtcgacttcttcaacaccatcgacgccgagaagatcacccagctgcccgtggtcaaggcctacgccctgtcctcgggtgcctc

  • Size:

    232

Documents

Citation Type Local copy
Scholtens, I., Laurensse, E., Molenaar, B., Zaaijer, S., Gaballo, H., Boleij, P., Bak, A., Kok, E. (2013) Practical Experiences with an Extended Screening Strategy for Genetically Modified Organisms (GMOs) in Real-Life Samples. J Agric Food Chem 61(38), 9097-109.Link to the document url. peer-reviewed article