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Detection Image Detection method details

Name:

 QL-ELE-Cry1F-F2/Cry1Fr-n2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence)

Comment:

 Target is the cry1F gene from bacterium Bacillus thuringiensis var. aizawai.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1F_tr-BACTA

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Cry1F-F2

  • Sequence:

    GACGTGGATCTTCATCTGCAATC

  • Size:

    23

  • Reverse Primer

  • Name:

    Cry1Fr-n2

  • Sequence:

    GCAACACGGCTGGCAATCG

  • Size:

    19

  • Probe

  • Name:

    Cry1F-P2

  • Sequence:

    CGCCCCCGGGATTGAAGACCCCGTAAC

  • Size:

    27

  • Amplicon:

  • Sequence:

    GACGTGGATCTTCATCTGCAATCCAGATGGCGCCCCCGGGATTGAAGACCCCGTAACTAGGAAAGTTGATACGATTGCCAGCCGTGTTGC

  • Size:

    90

Documents

Citation Type Local copy
Scholtens, I., Laurensse, E., Molenaar, B., Zaaijer, S., Gaballo, H., Boleij, P., Bak, A., Kok, E. (2013) Practical Experiences with an Extended Screening Strategy for Genetically Modified Organisms (GMOs) in Real-Life Samples. J Agric Food Chem 61(38), 9097-109.Link to the document url. peer-reviewed article