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Detection Image Detection method details

Name:

 QL-ELE-B'nase-F-n4/BNaseR-n3

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013).

Comment:

 Target is the barnase gene from bacterium Bacillus amyloliquefaciens.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-barnase

  • Oligonucleotides:

  • Forward Primer

  • Name:

    B'nase-F-n4

  • Sequence:

    TGGTACCGGTTATTCAACACG

  • Size:

    21

  • Reverse Primer

  • Name:

    BNaseR-n3

  • Sequence:

    GAGGGCTTGTGCTTCTGATTTT

  • Size:

    22

  • Probe

  • Name:

    BNaseP-n3

  • Sequence:

    GTCTGAAGATAATCCGCAACCC

  • Size:

    22

  • Amplicon:

  • Sequence:

    tggtaccggttattcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctc

  • Size:

    102

Documents

Citation Type Local copy
Scholtens, I., Laurensse, E., Molenaar, B., Zaaijer, S., Gaballo, H., Boleij, P., Bak, A., Kok, E. (2013) Practical Experiences with an Extended Screening Strategy for Genetically Modified Organisms (GMOs) in Real-Life Samples. J Agric Food Chem 61(38), 9097-109.Link to the document url. peer-reviewed article