
Detection method details
Name: |
QL-CON-p-nos-F2/NPTII-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) |
Comment: |
target is the junction region of a construct of the nopaline synthase promoter(P-nos) from Agrobacterium tumefaciens and the neomycin phosphotransferase II (nptII) gene from Escherichia coli (amplicon in event Ms1 is 151 bp) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 63-1, A1-DFR Petunias, B tomato, C5, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GS784, HLMT15-15, HLMT15-3, HLMT15-46, Huanong No. 1, INI-FMAPDF, KMD1, MPS965, Melon A, Melon B, Ms1, NF872, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, T120-7, Topas 19/2, X17-2
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Oligonucleotides:
Forward Primer
Name:
p-nos-F2Sequence:
TTCCCCTCGGTATCCAATTAGAGSize:
23
Reverse Primer
Name:
NPTII-RSequence:
GATTGTCTGTTGTGCCCAGTCASize:
22
Probe
Name:
NPTII-Tm2Sequence:
AGCCGAATAGCCTCTCCACCCAAGCSize:
25
-
Amplicon:
Sequence:
ttcccctcggtatccaattagagtctcatattcactctcaatccagatccggcccatgatcatgtggattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcSize:
151
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-CON-00-014![]() |
EU reference method protocol | 16-01-2020 |