
Detection method details
Name: |
QL-CON-PGS-bar-A2/PGS-bar-B2 |
Description: |
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13) |
Comment: |
Target is the junction region between the small subunit promoter (P-SSU) from Arabidopsis thaliana and the phosphinothricin acetyltransferase gene (bar) from Streptomyces hygroscopicus (BVL G30.40-13) |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3
|
-
Oligonucleotides:
Forward Primer
Name:
PGS-bar-A2Sequence:
gaagttgaccgtgcttgtctSize:
20
Reverse Primer
Name:
PGS-bar-B2Sequence:
caagtccaccaggcaagtaaSize:
20
-
Amplicon:
unknown
Documents
Citation | Type | Local copy |
---|---|---|
PCR detection of the pSSUAra-bar construct for screening of genetically modified oilseed rape lines - construct-specific method. BVL Official Method Collection G 30.40-13 Online Publication (2015)![]() |
other document | 04-05-2016 |