Detection method details
Name: |
QT-TAX-ZM-008 |
Description: |
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
Comment: |
Target is the endogenous high-mobility-group (hmg) gene from Zea mays. This assay is recommended for quantitative GMO analysis. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
-
Oligonucleotides:
Forward Primer
Name:
ZM1-FSequence:
TTGGACTAGAAATCTCGTGCTGASize:
23
Reverse Primer
Name:
ZM1-RSequence:
GCTACATAGGGAGCCTTGTCCTSize:
22
Probe
Name:
Probe ZM1Sequence:
CAATCCACACAAACGCACGCGTASize:
23
-
Amplicon:
Sequence:
ttggactagaaatctcgtgctgannnnnnnnnntacgcgtgcgtttgtgtggattgnaggacaaggctccctatgtagcSize:
79
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-008 | EU reference method protocol |