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Detection Image Detection method details

Name:

 QL-CON-00-004

Description:

 Qualitative conventional PCR method for detection of maize event Bt176 (ISO 21569)

Comment:

 Target is the junction region between the calcium-dependent protein kinase promoter (P-CDPK) from maize and the cryIA(b) gene

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 construct-specific

Target GMO names:
Bt176

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Cry03

  • Sequence:

    CTCTCGCCGTTCATGTCCGT

  • Size:

    20

  • Reverse Primer

  • Name:

    Cry04

  • Sequence:

    GGTCAGGCTCAGGCTGATGT

  • Size:

    20

  • Amplicon:

  • Sequence:

    ctctcgccgttcatgtccgtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacatcagcctgagcctgacc

  • Size:

    211

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-CON-00-004Link to the document url. EU reference method protocol