Detection method details
Name: |
QT-EVE-ZM-011 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of maize event T25 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-003 |
Type: |
event-specific |
Target GMO name: |
T25 |
-
Oligonucleotides:
Forward Primer
Name:
MLD143Sequence:
ACAAGCGTGTCGTGCTCCACSize:
20
Reverse Primer
Name:
MDB551Sequence:
GACATGATACTCCTTCCACCGSize:
21
Probe
Name:
TM016Sequence:
TCATTGAGTCGTTCCGCCATTGTCGSize:
25
-
Amplicon:
Sequence:
acaagcgtgtcgtgctccacnnnnnnnnnnnnnnnnnnnnnnnnntcattgagtcgttccgccattgtcgnnnnnnnnnnncggtggaaggagtatcatgtcSize:
102
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-011 | EU reference method protocol |