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Detection Image Detection method details

Name:

 QL-TAX-FMVorf7-F4/FMVorf7-R4

Description:

 Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012).

Comment:

 Target is the open reading frame VII (orf7) sequence of the figwort mosaic virus (FMV) genome.

Validation:

 in-house validation

Standardisation:

 national standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Figwort mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    FMVorf7-F4

  • Sequence:

    TATGCTAGGCTTAACGGGCTTC

  • Size:

    22

  • Reverse Primer

  • Name:

    FMVorf7-R4

  • Sequence:

    GGATCCGAGATTTTCTTGAAGTT

  • Size:

    23

  • Probe

  • Name:

    FMVorf7-FAM probe

  • Sequence:

    TCATCTCCAAGCGAGAATTTCAGCTGTTTC

  • Size:

    30

  • Amplicon:

  • Sequence:

    -

  • Size:

    113

Documents

Citation Type Local copy