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Detection Image Detection method details

Name:

 QL-ELE-00-015

Description:

 Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5).

Comment:

 Target is the Figwort mosaic virus 34S promoter (P-FMV), which in some GMOs is indicated as Figwort mosaic virus 34S enhancer (E-FMV).

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
E-FMV, P-34S FMV

  • Oligonucleotides:

  • Forward Primer

  • Name:

    pFMV-F

  • Sequence:

    CAAAATAACGTGGAAAAGAGCT

  • Size:

    22

  • Reverse Primer

  • Name:

    pFMV-R

  • Sequence:

    TCTTTTGTGGTCGTCACTGC

  • Size:

    20

  • Probe

  • Name:

    Probe pFMV

  • Sequence:

    CTGACAGCCCACTCACTAATGC

  • Size:

    22

  • Amplicon:

  • Sequence:

    caaaataacgtggaaaagagctnnnctgacagcccactcactaatgcnnnnnnnnnnngcagtgacgaccacaaaaga

  • Size:

    78

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-ELE-00-015Link to the document url. EU reference method protocol