Detection method details
Name: |
QT-ELE-00-001 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) |
Comment: |
Target is the Cauliflower mosaic virus 35S promoter (CaMV P-35S) |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-Cauliflower mosaic virus
|
-
Oligonucleotides:
Forward Primer
Name:
sFSequence:
CGTCTTCAAAGCAAGTGGATTGSize:
22
Reverse Primer
Name:
sRSequence:
TCTTGCGAAGGATAGTGGGATTSize:
22
Probe
Name:
35S coreSequence:
TCTCCACTGACGTAAGGGATGACGCASize:
26
-
Amplicon:
Sequence:
cgtcttcaaagcaagtggattgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaatcccactatccttcgcaagaSize:
79
Documents
Citation | Type | Local copy |
---|---|---|
Feinberg M., Fernandez S., Cassard S., Bertheau Y.; Quantitation of 35S promoter in maize DNA extracts from genetically modified organisms using real-time polymerase chain reaction, part 2: interlaboratory study J AOAC Int 88:558-573 (2005) | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QT-ELE-00-001 | EU reference method protocol |