Help icon

Detection Image Detection method details

Name:

 QT-TAX-zSSIIb-3F/zSSIIb-4R

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005)

Comment:

 Target is the endogenous starch synthase IIb (zSSIIb) gene from Zea mays.

Validation:

 unknown

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    zSSIIb-3F

  • Sequence:

    CGGTGGATGCTAAGGCTGATG

  • Size:

    21

  • Reverse Primer

  • Name:

    zSSIIb-4R

  • Sequence:

    AAAGGGCCAGGTTCATTATCCTC

  • Size:

    23

  • Probe

  • Name:

    zSSIIb-P

  • Sequence:

    TAAGGAGCACTCGCCGCCGCATCTG

  • Size:

    25

  • Amplicon:

  • Sequence:

    cggtggatgctaaggctgatgcagctccggctacagatgcggcggcgagtgctccttatgacagggaggataatgaacctggcccttt

  • Size:

    88

Documents

Citation Type Local copy
Yang L, Xu S, Pan A, Yin C, Zhang K, Wang Z, Zhou Z, Zhang D (2005) Event specific qualitative and quantitative polymerase chain reaction detection of genetically modified MON863 maize based on the 5’-transgene integration sequence. J Agric Food Chem. 53, 9312-9318Link to the document url. peer-reviewed article
Wang J, Xiao B, Zhang R, Guan H, Yang Y, Ding Y, Yuan R, Zhang X, Li L, Liang J, Wen H (2023) Inter-laboratory validation by event-specific qPCR methods for the detection of genetically modified insect and herbicide-tolerant maize DBN9501. Journal of Consumer Protection and Food Safety 18, 451-460Link to the document url. peer-reviewed article Download PDF pdf