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Detection Image Detection method details

Name:

 QT-TAX-ZM-008

Description:

 Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

Comment:

 Target is the endogenous high-mobility-group (hmg) gene from Zea mays. This assay is recommended for quantitative GMO analysis.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    ZM1-F

  • Sequence:

    TTGGACTAGAAATCTCGTGCTGA

  • Size:

    23

  • Reverse Primer

  • Name:

    ZM1-R

  • Sequence:

    GCTACATAGGGAGCCTTGTCCT

  • Size:

    22

  • Probe

  • Name:

    Probe ZM1

  • Sequence:

    CAATCCACACAAACGCACGCGTA

  • Size:

    23

  • Amplicon:

  • Sequence:

    ttggactagaaatctcgtgctgannnnnnnnnntacgcgtgcgtttgtgtggattgnaggacaaggctccctatgtagc

  • Size:

    79

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-008Link to the document url. EU reference method protocol