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Detection Image Detection method details

Name:

 QT-EVE-GM-019

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event DBN9004 (EU-RL GMFF GMOMETHODS database as of November 8, 2023).

Comment:

 Target is the 3' integration border region (IBR) between the insert of soybean event DBN9004 and the soybean host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-005

Type:

 event-specific

Target GMO name:

 DBN9004

  • Oligonucleotides:

  • Forward Primer

  • Name:

    S17A01_F

  • Sequence:

    GCCGTATCCGCAATGTGTTA

  • Size:

    20

  • Reverse Primer

  • Name:

    S17A01_R

  • Sequence:

    GCTCCATAAACGTGTGCTTTCA

  • Size:

    22

  • Probe

  • Name:

    S17A01_P

  • Sequence:

    TTGTTTACAACTCTGTGACCC

  • Size:

    21

  • Amplicon:

  • Sequence:

    gccgtatccgcaatgtgttannnnnnnnnnnnnnnnnnnnnttgtttacaactctgtgacccnnnnntgaaagcacacgtttatggagc

  • Size:

    89

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-019Link to the document url. EU reference method protocol