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Detection Image Detection method details

Name:

 QT-TAX-ZM-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the alcohol dehydrogenase 1 (adh1) gene from Zea mays

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    ADH-FF3

  • Sequence:

    CGTCGTTTCCCATCTCTTCCTCCT

  • Size:

    24

  • Reverse Primer

  • Name:

    ADH-RR4

  • Sequence:

    CCACTCCGAGACCCTCAGTC

  • Size:

    20

  • Probe

  • Name:

    ADH1-MDO

  • Sequence:

    AATCAGGGCTCATTTTCTCGCTCCTCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    cgtcgtttcccatctcttcctcctttagagctaccactatataaatcagggctcattttctcgctcctcacaggctcatctcgctttggatcgattggtttcgtaactggtgaaggactgagggtctcggagtgg

  • Size:

    135

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-003Link to the document url. EU reference method protocol