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Detection Image Detection method details

Name:

 QT-TAX-BN-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020)

Comment:

 Target is the cruciferin A (cruA) gene in oilseed rape (Brassica napus)

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Brassica napus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MDB510

  • Sequence:

    GGCCAGGGTTTCCGTGAT

  • Size:

    18

  • Reverse Primer

  • Name:

    MDB511

  • Sequence:

    CCGTCGTTGTAGAACCATTGG

  • Size:

    21

  • Probe

  • Name:

    TM458

  • Sequence:

    AGTCCTTATGTGCTCCACTTTCTGGTGCA

  • Size:

    29

  • Amplicon:

  • Sequence:

    ggccagggtttccgtgatntgcaccagaaagtggagcacataaggactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnccaatggttctacaacgacgg

  • Size:

    101

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-BN-003Link to the document url. EU reference method protocol