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Detection Image Detection method details

Name:

 QT-EVE-ZM-014

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event GA21 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-014

Type:

 event-specific

Target GMO name:

 GA21

  • Oligonucleotides:

  • Forward Primer

  • Name:

    esGA21-5' forward primer

  • Sequence:

    CGTTATGCTATTTGCAACTTTAGAACA

  • Size:

    27

  • Reverse Primer

  • Name:

    esGA21-5' reverse primer

  • Sequence:

    GCGATCCTCCTCGCGTT

  • Size:

    17

  • Probe

  • Name:

    esGA21-5' probe

  • Sequence:

    TTTCTCAACAGCAGGTGGGTCCGGGT

  • Size:

    26

  • Amplicon:

  • Sequence:

    cgttatgctatttgcaactttagaacannnnnnnnnnnnnnnnnntttctcaacagcaggtgggtccgggtnnnnnnnnnnnnnaacgcgaggaggatcgc

  • Size:

    101

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-014Link to the document url. EU reference method protocol