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Detection Image Detection method details

Name:

 QL-TAX-OS-006

Description:

 Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009).

Comment:

 target is the endogenous sucrose-phosphate synthase (SPS) gene of rice

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SPS-1F

  • Sequence:

    TTGCGCCTGAACGGATAT

  • Size:

    18

  • Reverse Primer

  • Name:

    SPS-2R

  • Sequence:

    GGAGAAGCACTGGACGAGG

  • Size:

    19

  • Amplicon:

  • Sequence:

    ttgcgcctgaacggatatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctcgtccagtgcttctcc

  • Size:

    277

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-OS-006Link to the document url. EU reference method protocol
Jiang L., Yang L., Zhang H., Guo J., Mazzara M., Van den Eede G., Zhang D.; International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified riceLink to the document url. peer-reviewed article