Detection method details
Name: |
QL-TAX-FMVorf7-F4/FMVorf7-R4 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012). |
Comment: |
Target is the open reading frame VII (orf7) sequence of the figwort mosaic virus (FMV) genome. |
Validation: |
in-house validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Figwort mosaic virus |
-
Oligonucleotides:
Forward Primer
Name:
FMVorf7-F4Sequence:
TATGCTAGGCTTAACGGGCTTCSize:
22
Reverse Primer
Name:
FMVorf7-R4Sequence:
GGATCCGAGATTTTCTTGAAGTTSize:
23
Probe
Name:
FMVorf7-FAM probeSequence:
TCATCTCCAAGCGAGAATTTCAGCTGTTTCSize:
30
-
Amplicon:
Sequence:
-Size:
113
Documents
Citation | Type | Local copy |
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