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Detection Image Detection method details

Name:

 QL-EVE-EC-002

Description:

 Qualitative real-time PCR (TaqMan) method for the detection of event PL73 (LM) (E. coli K-12 strain 19E) (EURL GMFF GMOMETHODS database as of November 24, 2017)

Comment:

 Target is the integration site of event PL73 (LM) corresponding to the 5’ flanking region of bacterial origin and the integration sequence inserted into the bacterial genome (insert to E. coli junction)

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 PL73 E. coli (LM)

  • Oligonucleotides:

  • Forward Primer

  • Name:

    LMA for

  • Sequence:

    GGTTATCCAGTAATAGCCATCTTCATC

  • Size:

    27

  • Reverse Primer

  • Name:

    LMA rev

  • Sequence:

    CCTCCCGGTTTTTTTCGTACTT

  • Size:

    22

  • Probe

  • Name:

    LMA probe

  • Sequence:

    CCGTCGCCGCTGTATTGATTCACTTG

  • Size:

    26

  • Amplicon:

  • Sequence:

    ggttatccagtaatagccatcttcatcnnnnnnnnnnccgtcgccgctgtattgattcacttgaagtacgaaaaaaaccgggagg

  • Size:

    85

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-EVE-EC-002Link to the document url. EU reference method protocol