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Detection Image Detection method details

Name:

 QL-ELE-00-024

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017).

Comment:

 Target is the E9 (T-E9) terminator from pea (Pisum sativum).

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 element-specific

Target DNA element:
T-rbcS_E9-PEA

  • Oligonucleotides:

  • Forward Primer

  • Name:

    T-E9-F

  • Sequence:

    TGAGAATGAACAAAAGGACCATATCA

  • Size:

    26

  • Reverse Primer

  • Name:

    T-E9-R

  • Sequence:

    TTTTTATTCGGTTTTCGCTATCG

  • Size:

    23

  • Probe

  • Name:

    T-E9-P

  • Sequence:

    TCATTAACTCTTCTCCATCCATTTCCATTTCACAGT

  • Size:

    36

  • Amplicon:

  • Sequence:

    tgagaatgaacaaaaggaccatatcattcattaactcttctccatccatttccatttcacagttcgatagcgaaaaccgaataaaaa

  • Size:

    87

Documents

Citation Type Local copy