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Detection method details
Name: |
QT-ELE-P35S 1-5'/P35S 1-3' |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) |
Comment: |
target is the 35S promoter (P-35S) sequence from the Cauliflower mosaic virus (CaMV) |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-Cauliflower mosaic virus
|
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Oligonucleotides:
Forward Primer
Name:
P35S 1-5'Sequence:
ATTGATGTGATATCTCCACTGACGTSize:
25
Reverse Primer
Name:
P35S 1-3'Sequence:
CCTCTCCAAATGAAATGAACTTCCTSize:
25
Probe
Name:
P35S-TaqSequence:
CCCACTATCCTTCGCAAGACCCTTCCTSize:
27
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Amplicon:
Sequence:
CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATSize:
100