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Detection Image Detection method details

Name:

 QT-TAX-FatA primer1/FatA primer2

Description:

 Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

Comment:

 Target is the endogenous acyl-acyl carrier protein thioesterase (FatA) gene of Brassica napus.

This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 taxon-specific

Target Species name:

 Brassica napus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    FatA primer1

  • Sequence:

    GGTCTCTCAGCAAGTGGGTGAT

  • Size:

    22

  • Reverse Primer

  • Name:

    FatA primer2

  • Sequence:

    TCGTCCCGAACTTCATCTGTAA

  • Size:

    22

  • Probe

  • Name:

    FatA probe

  • Sequence:

    ATGAACCAAGACACAAGGCGGCTTCA

  • Size:

    26

  • Amplicon:

  • Sequence:

    GGTCTCTCAGCAAGTGGGTGATGATGAACCAAGACACAAGGCGGCTTCAAAGAGTTACAGATGAAGTTCGGGACGA

  • Size:

    76

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article