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Detection Image Detection method details

Name:

 QT-EVE-OS-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of rice event LLRICE62 (EU-RL GMFF GMOMETHODS database as of January 01, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of rice event LLRICE62 and the rice host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-OS-017

Type:

 event-specific

Target GMO name:

 LLRICE62

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MDB616

  • Sequence:

    AGCTGGCGTAATAGCGAAGAGG

  • Size:

    22

  • Reverse Primer

  • Name:

    MDB694

  • Sequence:

    TGCTAACGGGTGCATCGTCTA

  • Size:

    21

  • Probe

  • Name:

    TM019

  • Sequence:

    CGCACCGATTATTTATACTTTTAGTCCACCT

  • Size:

    31

  • Amplicon:

  • Sequence:

    agctggcgtaatagcgaagaggcccgcaccgattatttatacttttagtccacctggtttttattgatagacgatgcacccgttagca

  • Size:

    88

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-OS-002Link to the document url. EU reference method protocol