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Detection Image Detection method details

Name:

 QT-EVE-GM-007

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of soybean event A5547-127 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-020

Type:

 event-specific

Target GMO name:

 A5547-127

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SHA003

  • Sequence:

    GCTATTTGGTGGCATTTTTCCA

  • Size:

    22

  • Reverse Primer

  • Name:

    SHA004

  • Sequence:

    CACTGCGGCCAACTTACTTCT

  • Size:

    21

  • Probe

  • Name:

    TM058

  • Sequence:

    CCGCAATGTCATACCGTCATCGTTGT

  • Size:

    26

  • Amplicon:

  • Sequence:

    gctatttggtggcatttttccannnnnccgcaatgtcataccgtcatcgttgtnagaagtaagttggccgcagtg

  • Size:

    75

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-007Link to the document url. EU reference method protocol
Delobel C., Bogni A., Mazzara M., Van den Eede G.; Event-specific Method for the Quantification of Soybean Line A5547-127 Using Real-time PCR - Validation Report and Protocol Online Publication (2009)Link to the document url. EU reference method protocol