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Detection Image Detection method details

Name:

 QT-EVE-GH-011

Description:

 Quantitative PCR method for detection of cotton event DAS81910 (EURL GMFF GMOMETHODS database as of January 10, 2019)

Comment:

 Target is the 5' integration border region (IBR) between the insert of cotton event DAS81910 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-016, QT-TAX-GH-021

Type:

 event-specific

Target GMO name:

 DAS81910

  • Oligonucleotides:

  • Forward Primer

  • Name:

    1706-f2

  • Sequence:

    AAGCTTAGGTGATTTCGATGATG

  • Size:

    23

  • Reverse Primer

  • Name:

    1706-r3

  • Sequence:

    GACCTCAATTGCGAGCTTTC

  • Size:

    20

  • Probe

  • Name:

    1706-p3

  • Sequence:

    CACACCAAAAGTTAGGCCCG

  • Size:

    20

  • Amplicon:

  • Sequence:

    aagcttaggt gatttcgatg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacac caaaagttag gcccgnnnnn nnnnnnnnnn gaaagctcgc aattgaggtc

  • Size:

    120

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-011Link to the document url. EU reference method protocol