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Detection Image Detection method details

Name:

 QT-EVE-BV-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of sugar beet event H7-1 (EU-RL GMFF GMOMETHODS database as March 13, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of sugar beet event H7-1 and the sugar beet host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BV-013

Type:

 event-specific

Target GMO name:

 H7-1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    H7PLT1

  • Sequence:

    TGGGATCTGGGTGGCTCTAACT

  • Size:

    22

  • Reverse Primer

  • Name:

    ZRH7-R2

  • Sequence:

    AATGCTGCTAAATCCTGAG

  • Size:

    19

  • Probe

  • Name:

    ZRH7

  • Sequence:

    AAGGCGGGAAACGACAATCT

  • Size:

    20

  • Amplicon:

  • Sequence:

    tgggatctgggtggctctaactnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaggcgggaaacgacaatctnnnnnnnnnnnnnnnnnnnctcaggatttagcagcatt

  • Size:

    108

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BV-001Link to the document url. EU reference method protocol