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Detection Image Detection method details

Name:

 QT-CON-00-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO/FDIS 21570).

Comment:

 Target is the junction region between the Cauliflower mosaic virus 35S promoter (CaMV P-35S) and the chloroplast transit peptide sequence from Petunia hybrida epsps gene (ctp4).

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QT-TAX-GM-002

Type:

 construct-specific

Target GMO names:
GTS 40-3-2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RR1-F

  • Sequence:

    CATTTGGAGAGGACACGCTGA

  • Size:

    21

  • Reverse Primer

  • Name:

    RR1-R

  • Sequence:

    GAGCCATGTTGTTAATTTGTGCC

  • Size:

    23

  • Probe

  • Name:

    RR1

  • Sequence:

    CAAGCTGACTCTAGCAGATCTTTC

  • Size:

    24

  • Amplicon:

  • Sequence:

    catttggagaggacacgctgacaagctgactctagcagatctttcnnnnnnggcacaaattaacaacatggctc

  • Size:

    74

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-CON-00-003Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document