Help icon

Detection Image Detection method details

Name:

 QL-ELE-Vip3A-F/Vip3A-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013).

Comment:

 Target is the vip3A gene from bacterium Bacillus thuringiensis strain AB88.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-vip3Aa20-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Vip3A-F

  • Sequence:

    TCACCAAGAAGATGAAGAC

  • Size:

    19

  • Reverse Primer

  • Name:

    Vip3A-R

  • Sequence:

    CTCTCCACCTTCTTCTTG

  • Size:

    18

  • Probe

  • Name:

    Vip3A-P

  • Sequence:

    GACCGCCAACTTCTACGACA

  • Size:

    20

  • Amplicon:

  • Sequence:

    -

  • Size:

    97

Documents

Citation Type Local copy
Liang et al., 2012