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Detection Image Detection method details

Name:

 QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD).

Comment:

 Target is the cry3Bb gene from bacterium Bacillus thuringiensis subsp. kumamotoensis.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry3Bb1-BACUK

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Cry3Bbf-n2

  • Sequence:

    CCGCCCAGGACTCCATCG

  • Size:

    18

  • Reverse Primer

  • Name:

    Cry3Bbr-n2

  • Sequence:

    GAGGCACCCGAGGACAGG

  • Size:

    18

  • Probe

  • Name:

    Cry3BbP-n3

  • Sequence:

    CTGCCGCCTGAGACCACTGACGAGC

  • Size:

    25

  • Amplicon:

  • Sequence:

    ccgcccaggactccatcgaccagctgccgcctgagaccactgacgagcccctggagaaggcctactcccaccagctgaactacgcggagtgcttcctgatgcaagaccgcaggggcaccatccccttcttcacctggacccaccgctccgtcgacttcttcaacaccatcgacgccgagaagatcacccagctgcccgtggtcaaggcctacgccctgtcctcgggtgcctc

  • Size:

    232

Documents

Citation Type Local copy
Scholtens et al. (2012)