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Detection Image Detection method details

Name:

 QT-EVE-ZM-017

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event LY038 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event LYO38 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 LY038

  • Oligonucleotides:

  • Forward Primer

  • Name:

    LY038 AF

  • Sequence:

    TGGGTTCAGTCTGCGAATGTT

  • Size:

    21

  • Reverse Primer

  • Name:

    LY038 AR

  • Sequence:

    AGGAATTCGATATCAAGCTTATCGA

  • Size:

    25

  • Probe

  • Name:

    LY038 AP

  • Sequence:

    CGAGCGGAGTTTATGGGTCGACGG

  • Size:

    24

  • Amplicon:

  • Sequence:

    tgggttcagtctgcgaatgttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncgagcggagtttatgggtcgacggnntcgataagcttgatatcgaattcct

  • Size:

    111

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-017Link to the document url. EU reference method protocol