Detection method details
Name: |
QT-EVE-GH-003 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON1445 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of cotton event MON1445 and the cotton host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GH-015 |
Type: |
event-specific |
Target GMO name: |
MON1445 |
-
Oligonucleotides:
Forward Primer
Name:
MON1445 primer forwardSequence:
GGAGTAAGACGATTCAGATCAAACACSize:
26
Reverse Primer
Name:
MON1445 primer reverseSequence:
ATCGACCTGCAGCCCAAGCTSize:
20
Probe
Name:
MON1445 probeSequence:
ATCAGATTGTCGTTTCCCGCCTTCAGTTTSize:
29
-
Amplicon:
Sequence:
ggagtaagacgattcagatcaaacacnnnnnnnnnaaactgaaggcgggaaacgacaatctgatnnnagcttgggctgcaggtcgatSize:
87
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-003 | EU reference method protocol |